Determination of enantiomers in R-lansoprazole by HPLC
GE lin
LIU Jia
ZHAO Jie
ZHONG Liu-yi
Abstract：Objective:To establish a high performance liquid chromatography(HPLC)method for determination of S-lansoprazole in R-lansoprazole.Methods:CHIRALPAK IC column(250 mm×4.6 mm,5 μm)was used,with n-hexane-absolute ethanol-ethylenediamine(80∶20∶0.1)as the mobile phase at the detection wavelength of 285 nm,with a flow rate of 0.8 mL·min-1.The column temperature was maintained at 30℃.Results:The calibration curves of R-lansoprazole and S-lansoprazole were linear in the range of 0.287～15.660 and 0.183～15.648 μg·mL-1,respectively,and the correction factor was 1.0.The average recovery was 97.8%with RSD of 1.5%(n=9).The S-lansoprazole could be well separated form S-lansoprazole.Conclusion:This method is highly specific,sensitive,accurate,and reproducible,which is suitable for the determination of S-lansoprazole in R-lansoprazole.
Keywords：R-lansoprazoleS-lansoprazoleHPLC
Publication Date：2026-03-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 627-631 )