Establishment and application of a detection method for ristocetin cofactor activity of human von Willebrand factor
MA Qiu-ping
WANG Min-li
WANG Qing-zhou
Abstract：Objective:To establish and validate a method of measuring the ristocetin activity in human von Willebrand factor(vWF)products and preliminarily apply this method.Methods:The international standard of human vWF(lot 09/182)was diluted to create a standard curve.An automated coagulometer was used to detect the ristocetin activity in human vWF products by immunoturbidimetry.The quantitative response relationship and detection range were determined.The specificity,linear range,repeatability,and accuracy of the method were validated.Then this method was applied to detect ristocetin activity in plasma-derived and recombinant vWF products.Results:The method exhibited good specificity.The ristocetin activity of the international standard showed a good third-order polynomial linearity within the range of 0.1～0.6 IU·mL-1,with a correlation coefficient above 0.99.The repeatability results showed that the RSD was less than 10%,and the spike recovery rate was between 80.9%～110.0%.Conclusion:This method can rapidly and accurately measure the ristocetin activity in human vWF products and can be used for the quality control of ristocetin activity in such products.
Keywords：human von Willebrand factorvon Willebrand diseaseblood productsimmunoturbidimetryautomated coagulometer
Publication Date：2026-01-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 200-204 )