Quality evaluation of Codonopsis pilosula Oral Liquid based on HPLC fingerprint and content determination
SUN Ni-ni
LIU Cai-xia
MO Ya-dan
DU Chen-hui
DONG Lu-lu
PEI Xiang-ping
Abstract：Objective:To establish an HPLC fingerprint of Codonopsis pilosula Oral Liquid and simultaneously determine the contents of Syringin,Codonopsis alkynin,Codonopsis alkynyl glycoside,Codonopsis pilosula alkynyl alcohol,Atractylodes lactone Ⅲ for quality evaluation.Methods:Chromatographic separation was performed on a DiKMAC18 column(250 mm×4.6 mm,5 μm)using a gradient elution of acetonitrile and 0.1％acetic acid solution as the mobile phase.The flow rate was1.0mL·min-1,the column temperature was maintained at25℃,detection wavelengths were set at 220,215,and 267 nm,and the injection volume was 20 μL.Results:The established fingerprint showed 10 common peaks,with similarity values among different batches exceeding 0.981.The contents of Syringin,Codonopsis alkynin,Codonopsis alkynyl glycoside,Codonopsis pilosula alkynyl alcohol and Atractylodes lactone Ⅲ were 0.032～0.054,0.030～0.041,0.066～0.112,0.006～0.013,0.005～0.010 mg·mL-1,respectively.The five compounds exhibited good linearity(r≥0.9991)within their respective concentration ranges.The relative standard deviations(RSDs)for precision,repeatability,and 24-h stability were all below 3.000％.The average recoveries ranged from 97.701％to 101.636％,with RSDs between 1.347％and 2.551％.Conclusion:This method is stable,scientifically sound and reproducible,providing a scientific basis for improving the quality evaluation method of Codonopsis pilosula Oral Liquid.
Keywords：Codonopsis pilosula Oral LiquidHPLCfingerprintquantitative analysis of multi-index componentsquality evaluation
Publication Date：2026-01-15
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 93-99 )