Inhibitory effect and mechanism of IgD-Fc-Ig fusion protein on human rheumatoid cell model
LING Xi
XU Jing
LI Chao-yuan
XIE Jin-xiang
CHEN Meng-qin
LIU Dan-yan
DONG Man-ling
WU Tian-tian
WEI Wei
WU Yu-jing
Abstract：Objective:To explore the mechanism of action of IgD-Fc-Ig fusion protein on a human rheumatoid cell model,which involves co-culturing FcδR high/low expressing Jurkat cells(Jurkat-WT,Jurkat-FcδR+,Jurkat-FcδR+/-)with MH7A cells following immunoglobulin D(IgD)stimulation.Methods:In vitro experiments were set up with a blank control group,an IgD group,and an IgD-Fc-Ig treatment group.The effects of IgD-Fc-Ig on the proliferation of MH7A cells in the human rheumatoid cell model were observed using CCK-8 cell viability assay kit and EDU-488 cell proliferation assay kit.The impact of IgD-Fc-Ig on the invasion and migration of MH7A cells in the human rheumatoid cell model was assessed using Transwell chambers.Results:In the human rheumatoid cell model,Jurkat-FcδR+cells exhibited stronger promotion of proliferation,migration,and invasion of MH7A cells compared to Jurkat-WT and Jurkat-FcδR+/-cells.IgD-Fc-Ig was found to inhibit the proliferation,migration,and invasion effects of Jurkat-WT and Jurkat-Fc8R+cells on MH7A cells in the human rheumatoid cell model.Conclusion:IgD-Fc-Ig can target high levels of Fc8R,thereby inhibiting the abnormal activation of fibroblast-like synoviocytes and the cartilage-destructive effects induced by IgD-stimulated T cells.
Keywords：rheumatoid arthritisT cellfibroblast-like synoviocyteimmunoglobulin DIgD-Fc-Ig
Publication Date：2025-11-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：8( 2443-2450 )