Using DRS origin software to establish an HPLC surrogate reference standard method for the determination of cimicifugoside,5-O-methylvisammioside,cimicifugin and hamaudol glycoside in Saposhnikovia divaricata
KONG De-juan
ZUO Tian-tian
SHENG Xue
JI Zeng-yun
ZOU Guang-xin
TIAN Jia-xun
WANG Yan-nan
YAN Feng-jie
Abstract：Objective:To establish a two reference substances for determination of multiple component(TRSDMC)method for simultaneous determination of the contents of cimicifugoside,5-O-methylvisammioside,cimicifugin,hamaudol glycoside in Saposhnikovia divaricata,this study aims to enhance quality control of this variety while saving reference standards.Methods:HPLC method was employed.A C18 column was utilized with a mobile phase consisting of acetonitrile(A)-0.1%formic acid in water(B),at a flow rate of 1 mL·min-1 using gradient elution.The column temperature was maintained at 35℃,with a detection wavelength set at 254 nm and an injection volume of 10 μL.Cimicifugoside and 5-O-methylvisammioside were used as controls;DRS origin software facilitated linear calibration with two reference substances to predict the retention times of characteristic peaks.The relative correction factor was obtained by replacing the reference method,and the result were compared with the external standard method.Results:The standard curve of the analytes predicted by DRS origin software was accurate,and the contents calculated by combining the correction factors were compared with the result of the external standard method.The absolute deviation was<0.2 mg·g-1,and the accuracy was>95.0%.The content ranges of cimicifugoside,cimicifugin,5-O-methylvisammioside and hamaudol glycoside in 24 samples were 0.839 9~3.799 6,0.020 6~0.1718,0.677 3~2.509 9,0.045 7~0.203 2 mg·g-1.Conclusion:Compared with the relative retention time method,the dual-standard linear correction for predicting retention time offers higher accuracy in retention time prediction and a broader applicability range for chromatographic columns.This study provides a new approach and methodology for the multi-index content determination of Saposhnikovia divaricata.
Keywords：Saposhnikovia divaricatacimicifugosidecimicifugin5-O-methylvisammiosidehamaudol glycosidealternative reference methodDRS origin software
Publication Date：2025-11-15
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：10( 2316-2325 )