Correlation between quantity-quality changes of characteristic components in different ratios of Salviae Miltiorrhizae Radix et Rhizoma-Epimedii Folium
QI Yu-tong
MENG Shuo
PENG Qing
LIN Li
REN Jun-guo
ZHANG Miao
LIU Jian-xun
Abstract：Objective:To explore the quantitative-qualitative changes of the herb pair with different ratios based on specific chromatogram,network pharmacology,and spectral-effect correlation analysis to provide a reference for the quantity-effect correlation and clinical application of herb pair.Methods:HPLC method was used to establish the characteristic fingerprint of herb pairs with different ratios.Network pharmacology was introduced to obtain the relationship between key targets and pathways of herb pair on the antioxidants of characteristic components.The antioxidant activity of seven batches of herb pair was evaluated by the DPPH and ABTS radical scavenging methods,and partial least squares regression(PLSR)was applied to analyze the spectrum-effect relationship between fingerprint and antioxidant activity,selecting the active ingredients,which was finally determined by HPLC.Results:A total of 14 common peaks were identified in the fingerprint with a similarity higher than 0.80.Network pharmacology confirmed that epimedin C and tanshinone Ⅰ were the active components.They might exert their antidepressant effect through the core targets including ESR1,PIK3CA,and HDAC1.The herb pair with different ratios had strong scavenging abilities of DPPH and ABTS.The OPLS-DA model could successfully distinguish seven proportions of herb pairs,among which epimedin C,tanshinone I,lithospermic acid,and salvianolic acid B were differential markers in them.The content measurement results showed that there were significant differences in the characteristic components compared with DS and YYH.Conclusion:When DS and YYH are mixed in different proportions,the correlation between the quality indicated by the contents of characteristic ingredients and antioxidant activity and the quantity used for compatibility is obvious,which provided a scientific basis for the rational use of drugs in clinical practice.
Keywords：Salviae Miltiorrhizae Radix et Rhizoma-Epimedii Foliumdrug pairing ratiofingerprintingnetwork pharmacologyantioxidantspectral-effect relationship
Publication Date：2025-09-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：11( 1974-1984 )