The study of pogostemonis herba,pogostemonis herba leaves,and pogostemonis herba stalks by HPLC fingerprint and multi-index component determination
GAO Le
ZHEN Ya-qin
ZHANG Ze-zhao
XIE Ze-yang
TIAN Wei
NIU Li-ying
Abstract：Objective:To establish an HPLC method for simultaneous determination of fingerprints and 10 components of pogostemonis herba,pogostemonis herba leaves,and pogostemonis herba stalks,and analyze the differences of chemical components among the three components,so as to provide reference for improvement of quality control methods of pogostemonis herba.Methods:The chromatography was performed by HPLC with Agilent Poroshell 120EC-C18 column(250 mm×4.6 mm,4.0 μm).The mobile phase was acetonitrile-0.1%phosphoric acid solution,gradient elution was performed at a flow rate of 1.0 mL·min-1,and the column temperature was maintained at 35℃.The detection wavelength was 330 nm.Results:The similarity between different batches of pogostemonis herba,pogostemonis herba leaves,pogostemonis herba stalks and their control fingerprint was greater than 0.91.Ten chromatographic peaks were identified by reference substances.The similarity indexes between pogostemonis herba leaves control,pogostemonis herba stalks and pogostemonis herba control fingerprint were 0.75 and 0.95,respectively.The similarity between leaves and stalks of pogostemonis herba was only 0.57.The method of content determination of 10 components met the requirements of methodological investigation.The contents of isomonitroside and pogostemonis herba ketone in pogostemonis herba stalks were higher than those in pogostemonis herba leaves,and the contents of other eight components were higher in pogostemonis herba leaves.Conclusion:The fingerprint and content determination method established in this study are simple,stable and reliable,and provide a reference for further improving the quality standard of pogostemonis herba.
Keywords：pogostemonis herbapogostemonis herba leavespogostemonis herba stalksfingerprintcontent determinationquality control
Publication Date：2025-08-03
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：8( 1767-1774 )