Establishment of fingerprint and the analysis of chemical composition of Hypericum kouytchense Lévl.using UHPLC-DAD and UHPLC-Q-Exactive Plus Orbitrap HRMS
HE Yu
YANG Bo
SHAO Fei-lin
SUN Fei
SUN Jia
LU Yuan
PAN Jie
HUANG Yong
LI Yong-jun
LIU Chun-hua
Abstract：Objective:To establish the fingerprint chromatograms of Hypericum kouytchense L.and to analyze its chemical composition.Methods:The fingerprint chromatograms of Hypericum kouytchense L.were developed using UHPLC-DAD.Similarity analysis,cluster analysis,and principal component analysis were performed on the fingerprint chromatograms of 12 batches of samples.Chemical constituents were tentatively identified using UHPLC-Q-Exactive Plus Orbitrap HRMS.Results:The fingerprint chromatogram analysis revealed that the similarity among the 12 batches of Hypericum kouytchense L.ranged from 0.928 to 0.999.Cluster analysis and principal component analysis indicated that there might be certain differences in the relative contents of main compounds in the samples from different sources.Additionally,a total of 69 compounds,including flavonoids,xanthones,and organic acids,were tentatively identified in Hypericum kouytchense L.Conclusion:This method is stable and reliable,effectively assessing the quality of Hypericum kouytchense L.,providing a foundation for its further development and utilization.
Keywords：Hypericum kouytchense Lévl.UHPLC-DADUHPLC-Q-Exactive Plus Orbitrap HRMSfingerprintschemical composition
Publication Date：2025-08-15
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 1666-1672 )