Simultaneous determination of four components in vitamin B complex injection by HPLC
GENG Ying
HUANG Hui-fen
WEI Ning-yi
CHEN Hua
Abstract：Objective:To establish an HPLC method for simultaneous determination of vitamin B1,vitamin B2,vitamin B6 and nicotinamide in vitamin B complex injection.Methods:The determination was performed on a Waters XBridge C18 column(250 mm × 4.6 mm,5 μm)with mobile phase consisting of methanol-0.054 mol·L-1 potassium dihydrogen phosphate solution at a flow rate of 1.0 mL·min-1 for gradient elution.The detection wavelength was set at 275 nm.The column temperature was maintained at 35 ℃,and the injection volume was 20 μL.Results:The line ranges of vitamin B1,vitamin B2,vitamin B6 and nicotinamide were 20.138～402.761 μg·mL-1(r=0.9999),2.045～40.890 μg·mL-1(r=0.999 9),2.038～40.760 μg·mL-1(r=0.999 9)and 31.219～624.375 μg·mL-1(r=0.999 9),respectively.The recoveries were 100.4％～101.1％(RSD=0.3％),99.0％～101.5％(RSD=0.8％),98.2％～102.1％(RSD=1.2％),and 100.2％～101.7％(RSD=0.6％)(n=9),respectively.RSDs of precision,stability and reproducibility were all below 1.0％(n=6).The sample solution was stable at room temperature over a period of 48 h.Conclusion:The method is proved rapid,accurate and convenient,which can be used in the quality control of vitamin B complex injection,for the determination of four components in vitamin B complex injection simultaneously.
Keywords：vitamin B complex injectionHPLCquality controlriboflavin sodium phosphate(content calculated as vitamin B2)vitamin B1vitamin B6niacotinamide
Publication Date：2025-08-15
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 1647-1653 )