Rapid qualitative and quantitative analysis of perilla leaf and white perilla leaf based on an ultra-high performance convergence chromatography

NIE Li-xing; HUANG Lie-yan; WANG Xin-pin; XUE Dan; KANG Shuai; WEI Feng

doi:10.20251/j.cnki.1003-3734.2025.13.011

Abstract：Objective:A qualitative and quantitative analysis method for perilla leaf and white perilla leaf was established using ultra-performance convergence chromatography(UPC2).Methods:The samples were extracted by methanol.The separations were performed on Waters TorusTM DIOL(100 mm×3.0 mm,1.8 μm)and Waters Viridis HSS C18 SB(100 mm×3.0 mm,1.7 μm)columns using CO2(100%)and CO2(A)-95%methanol containing 0.1%trifluoroacetic acid(B)(80∶20)as the mobile phase,respectively.The flow rate was 0.8mL·min-1,the column temperature was maintained at 43℃and the system back pressure was 1800 psi.The injection volume was 1 μL.Cluster analysis was conducted on the chromatograms of perilla leaf with both surfaces purple(PP),perilla leaf with upper surface green and the lower surface purple(GP),and white perilla leaf with both surfaces green(GG).And the contents of perillaldehyde and rosmarinic acid in these samples were determined.Results:Different characteristics were discovered in the chromatograms of PP,GP,and GG.Chemical composition of perilla leaf with both surfaces purple and white perilla leaf with both surfaces green showed greater difference.Perillaldehyde and rosmarinic acid had a good linear relationship within their respective ranges.Relative standard deviations for precision,repeatability and stability tests were all below 3%.The average recoveries were 98.20%and 97.82%,respectively.Conclusion:Using UPC2 as single instrument,this method successfully achieved both qualitative and quantitative analyses of non-volatile and volatile components within 4 min through coordinating columns with different polarities.The method is fast,organic solvent saving,and environmental friendly.This study can provide scientific basis for the use and quality control of perilla leaves,as well as new idea and reference for the application of UPC2 in analysis of traditional Chinese medicine.