Research of carcinogenic risk assessment for Pb and As in pheretima and scorpio based on in vitro PBET digestion/Caco-2 cell model
ZUO Tian-tian
XIE Si-min
JIN Hong-yu
LIU Jing
CHENG Xian-long
WEI Feng
Abstract：Objective:To determine the contents of Pb and As in pheretima and scorpio by inductively coupled plasma mass spectrometry(ICP-MS)and establish in vitro physiologically-based extraction test(PBET)digestion/Caco-2 cell model to investigate the bioaccessibility of Pb and As.Methods:Carcinogenic risk(CR)method was used to assess the cancer risks owing to exposure to Pb and As.Results:After transportation by Caco-2 cell,the bioaccessibility values of Pb and As in pheretima were 6.21%～25.37%and 29.33%～65.36%,respectively,and were 26.89%～74.23%and 18.98%～56.78%in scorpio,respectively.The risk assessment results revealed that based on the total amount of heavy metals,the CR values of As in seven batches of pheretima were higher than 10-4,indicating a high cancer risk.After cell transportation,the CR values of Pb and As in all batches of pheretima and scorpio were below 10-4,indicating an acceptable cancer risk.Conclusion:This study establishes a novel model for risk assessment of heavy metals in pheretima and scorpio based on bioaccessibility,which provides a technical support for scientific and objective assessment of the health risks of heavy metals in traditional Chinese medicines and lays the foundation for the establishment of limit standard.
Keywords：pheretimascorpioin vitro physiologically-based extraction test digestion/Caco-2 cell modelPbAscarcinogenic risk assessment
Publication Date：2025-06-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 1289-1295 )