Determination of trilaciclib in rat plasma by LC-MS/MS and its pharmacokinetic study
CHENG Tian
XIE Wei-wei
ZHANG Yu-qian
MA Ying-hua
DU Ying-feng
Abstract：Objective:To establish a rapid,sensitive and convenient LC-MS/MS method for determination of trilaciclib in rat plasma and investigate its pharmacokinetic characteristics.Methods:The plasma samples were pretreated by protein precipitation method with organic solvent,and acetaminophen was used as internal standard.The analytes were separated on a Thermo hypersil goldtmTM column(50.0 mm×2.1 mm,3 μm),and the mobile phase was gradiently eluted with 0.1%formic acid-water(A)and acetonitrile(B)at a flow rate of 0.45mL·min-1.Positive ion mode of electrospray ion source and multiple reaction monitoring were adopted.The ion pairs of trilaciclib and internal standard were m/z 447.2→336 and m/z 152→110.1,respectively.Results:Trilaciclib was not affected by plasma matrix,and had a good linear relationship between 10～5 000 ng·mL-1(r=0.995 07),with the lower limit of quantification being 10 ng·mL-1.The intra-assay and inter-assay precision RSDs were≤9.45%,with good stability.The methodological validation was in line with the provisions of Chinese Pharmacopoeia(2020 Edition).Conclusion:The method is rapid,simple,and specific,which is suitable for investigating the pharma-cokinetic characteristics of trilaciclib in rats,which provides a methodological reference for the in-depth clinical study of trilaciclib,a new CDK4/6 inhibitor.
Keywords：trilaciclibLC-MS/MSplasma drug concentrationpharmacokineticsCDK4/6 inhibitors
Publication Date：2025-05-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：6( 1110-1115 )