Progress in the application of in vitro models for teratogenicity research in drug development
FANG Ya-li
LI Shen-ning
ZHOU Chang-hui
CUI Wen-teng
FENG Tian-yi
CHANG Yan
Abstract：With the global attention to animal welfare and the progress of science and technology,more efficient and accurate in vitro models for teratogenicity evaluation have gradually emerged.These models include embryonic stem cell test(Embryonic Stem Cell Trials,EST),microculture test(Microcarrier Culture Trials,MCT),whole embryo culture test(Whole Embryo Culture Test,WEC),and the models based on human induced pluripotent stem cells(Human Induced Pluripotent Stem Cells,hiPSC).They provide a methodological supplement for in vivo embryonic developmental toxicity testing.In particular,hiPSCs,with the ability to differentiate into all cell types in vivo,have become a key model for constructing complex embryonic development models,simulating specific developmental stages and conducting directed organ differentiation studies.With the development of three-dimensional cell culture technology,organoid system,high-throughput screening and organ chip technology,in vitro models have made remarkable progress in simulating human organ microenvironment,co-culture of multiple cell types and reconstruction of organ 3D structure and function on micro-platform.These advances not only improve the efficiency of drug screening,but also enhance our understanding of how drugs interfere with embryonic development.Although the current models still face challenges of physiological relevance,complexity,and ethics,they show great potential in assessing drug safety.
Keywords：vitro modelteratogenicitydevelopmental toxicitytoxicity evaluationinduced pluripotent stem cells
Publication Date：2025-05-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 1063-1069 )