Preparation and evaluation of folic acid-modified cantharidin and staurosporine co-loaded liposomes
ZHONG Zi-yi
WEI Ai-qiu
HE Jin-cheng
CHEN Ling-li
ZHANG Xiao-qing
Abstract：Objective:To prepare a folic acid-modified liposomes nanodelivery system co-loaded with cantharidin and staurosporine(FA/CTD-STS/NL)and evaluate its properties.Methods:The FA/CTD-STS/NL formulation was prepared using thin film dispersion method,and the encapsulation efficiency of liposomes was determined by gel column-HPLC method.The morphology of liposomes was observed by transmission electron microscopy(TEM).The particle size,distribution and Zeta potential of liposomes were measured by laser particle size analyser,and the serum stability and long-term stability were investigated.The haemocompatibility of FA/CTD-STS/NL was determined by microplate reader,while the cytotoxicity was detected by CCK-8 assay.Results:FA/CTD-STS/NL was spherical in appearance and uniformly dispersed.The average particle size,PDI,and Zeta potential were(114.3±0.9)nm,(0.212±0.018)and-(1.19±0.13)mV,respectively.The average encapsulation rate was (89.62±0.67)％ and (96.01±1.02)％,respectively. The serum stability and long-term stability were good. The hemolysis rate was less than 5％,indicating good blood compatibility. FA/CTD-STS/NL showed good cytocompatibility with mouse normal hepatocytes (AML-12 cells). Conclusion:The prepared FA/CTD-STS/NL had good stability and biosafety,with high entrapment efficiency and particle size in line with the requirements of anticancer nanodelivery carriers,which is conducive to drug enrichment in tumor tissues. This study lays the experimental foundation for subsequent studies on targeting,anti-hepatocarcinoma effect and mechanism in vivo and in vitro.
Keywords：folic acidcantharidinstaurosporineliposomesnanodelivery system
Publication Date：2025-05-15
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：8( 978-985 )