Research progress of chitosan as carriers of nucleic acid in gene therapy
LI Hua-an
LI Zhi-yang
LIN Bo-han
LI Han-qi
WEN Ding-sheng
WEN Lu
CHEN Gang
Abstract：As a type of cationic polymer,chitosan is an ideal carrier for nucleic acid delivery due to its excellent biocompatibility,remarkable biodegradability and high cation density.The structure of chitosan contains a large number of amino groups,and the transfection efficiency of nucleic acid is affected by several factors like deacetylation degree,molecular weight,pH,serum and N/P ratio.Due to its superior delivery abilities,chitosan has been widely used in the delivery of DNA and RNA.In addition,chitosan is also used to deliver CRISPR/Cas9 for gene editing,which is a novel approach to disease treatment.Nevertheless,chitosan and its derivatives as gene carriers still face obstacles at both intracellular and extracellular levels that necessitate further resolution.
Keywords：chitosancationic polymernucleic acid deliverygene therapygene editing
Publication Date：2025-04-15
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 713-719 )