Simultaneous determination of ten chemical components in Menggen Vos-18 pills by method of quantitative analysis of multi-components by single marker
SU Duna-buqi
HE Ye
MO Ri-gen
HAO Jun-sheng
HAI Jin
WANG Xiu-lan
Abstract：Objective:To establish an HPLC method for simultaneous determination of 10 components(gallic acid,epicatechin,ellagic acid,myricetin,cinnamic acid,eugenol,hesperidin,alpha-asarol and chrysophanol)in a Mongolian medicine,Menggen Vos-18 pills,and verify the feasibility of applying this method in its quality analysis.Methods:The chromatography was performed on Agilent Eclipse Plus C18 column(250 mm×4.6 mm,5 μm)with mobile phase consisting of acetonitrile(A)-0.2% phosphoric acid solution(B)and gradient elution(0～60 min,5% A→25% A,wavelength 210 nm;60～80 min,25% A→36% A,wavelength 280 nm;80～145 min,36% A→75% A,wavelength 254 nm),and the flow rate was 1.0 mL·min-1.The column temperature was 40℃.Relative correction factors(fs/i)were established with ellagic acid as the internal reference,the mass fractions of each component were calculated by relative correction factor,and the results were determined by external standard method.The accuracy of the application of quantitative analysis of multi-components by single marker(QAMS)method in Menggen Vos-18 pills was evaluated.Results:The fs/i reproducibility of gallic acid,epicatechin,myricetin,cinnamic acid,eugenol,hesperidin,manpinone,alpha-asarol and chrysophenol in Menggen Vos-18 pills was good.They were 0.2422,0.2315,0.3199,0.2565,1.9699,1.2089,1.2602,0.5605 and 1.2132,respectively.The results obtained by QAMS method and external standard method were close to each other.Conclusion:The method is simple,stable and reproducible,and can be used for the quality control of Mengen Vos-18 pills.
Keywords：Menggen Vos-18 pillsrelative correction factorquantitative analysis of multi-components by single markergallic acidepicatechinellagic acidmyricetincinnamic acideugenolhesperidinmancinonealpha-asarolchrysophanol
Publication Date：2025-02-27
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 419-425 )