The application of error-corrected duplex sequencing in genetic toxicity and carcinogenicity
CUI Wen-teng
ZHENG Ming-lan
ZHOU Chang-hui
HE Wei-wei
FANG Ya-li
FENG Tian-yi
DU Hong-li
CHANG Yan
Abstract：Error correction is crucial for next-generation sequencing applications,as highly accurate sequencing readings can yield higher quality results.Error-corrected double sequencing(ecNGS)based on consensus sequencing can directly quantify low-frequency mutations and provides enormous potential for chemical mutagenicity assessment,rapidly becoming a valuable,highly sensitive,and accurate method.Recent studies on mutagenicity and carcinogenicity have used ecNGS to quantify chemically-induced mutations and mutation profiles associated with cancer risk,which plays an important role in genetic toxicity testing and carcinogenicity assessment.The three common methods of ecNGS have different application prospects in genetic toxicity and carcinogenicity detection.In addition,ecNGS has also played an important role in human-related regulation.Finally,prospects for the application of ecNGS were proposed,which could further develop ecNGS technology and include its endpoints in non-clinical safety studies to supplement or provide references for replacing current testing methods in some cases.
Keywords：error-corrected duplex sequencingsequencinggenetic toxicitycarcinogenicitymutagenicity
Publication Date：2025-02-27
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 394-400 )