Simultaneous determination of five constituents in Wuwei Ganlu Yaoyu decoction powder by HPLC
DING Yin
WANG Ya-qian
LUO Yu-ting
GOU Xiao-ling
WANG Tian-hong
FAN Gang
Abstract：Objective:To develop an HPLC method for simultaneous determination of five components(gallic acid,hyperoside,isochlorogenic acid A,quercitrin,and isochlorogenic acid C)in the Tibetan medicine Wuwei Ganlu Yaoyu decoction powder.Samples from six different sources were determined and analyzed.Methods:The SWELL Chromplus C18 column(250 mm ×4.6 mm,5 μm)was used.A mobile phase consisting of acetonitrile and 0.2％phosphoric acid solution was used for gradient elution.The detection wavelength was set at 254 nm.Results:The linear ranges of gallic acid,hyperoside,isochlorogenic acid A,quercitrin,and isochlorogenic acid C were 24.45～195.6,8.55～68.4,10.9～87.2,11～88 and 15.86～126.8 μg·mL-1 respectively.The RSD of precision,stability,and repeatability were all less than 3％.The average spiked recoveries of the five components ranged 99.60％～104.03％with RSDs of 2.68％～1.13％.The contents of the five components in the samples from different sources ranged 145～1 381,0～441,62～230,26～166,and 107～363 µg·g-1.Conclusion:The established HPLC method is simple and reproducible,and it can be used for the quality control and evaluation of Wuwei Ganluo Yaoyu decoction powder.
Keywords：Wuwei Ganlu Yaoyu decoction powdercontent determinationHPLCquality control
Publication Date：2025-02-14
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 317-321 )