Determination of puerarin,hesperidin,ginsenoside Rb1,and ammonium glycyrrhizinate in Shufengzaizao Pills by UPLC-CAD
HAO Yun-fang
DU Juan
SHI Xian-hai
YANG Yan
ZHENG Zhe
LIAN Yun-lan
Abstract：Objective:To develop a UPLC-CAD method for simultaneous determination of puerarin,hesperidin,ginsenoside Rb1,and ammonium glycyrrhizinate in Shufengzaizao Pills.Methods:UPLC analysis was performed on a Waters Atlantis C18 column(150 mm×2.1 mm,3 μm),with mobile phase consisting of acetonitrile(A)-0.1%formic acid solution(B)at a flow rate of 0.3 mL·min-1(0～60 min,95%～10%B),and the column temperature was 35℃.The injection volume was 2 μL.The nebulizer chamber temperature for the CAD system was maintained at 35℃,the data acquisition frequency was 5 Hz,and the filter constant F was 3.6 s.Results:The UPLC-CAD approach showed good linearity for puerarin,hesperidin,ginsenoside Rb1,and ammonium glycyrrhizinate,and the correlation coefficients for calibration curves were both more than 0.9990.The RSD of precision,repeatability,and stability in 24 h were all less than 3.0%.The average recovery rates of puerarin,hesperidin,ginsenoside Rb1,and ammonium glycyrrhizinate were 106.51%,99.81%,105.77%,and 103.82%,respectively.Conclusion:The established UPLC-CAD method can be used for the simultaneous determination of puerarin,hesperidin,ginsenoside Rb1,and ammonium glycyrrhizinate in Shufengzaizao Pills,which provides a new approach for the analysis and quality control of Shufengzaizao Pills.
Keywords：Shufengzaizao pillsUPLC-CADpuerarihesperidinginsenoside Rb1ammonium glycyrrhizinatequantitative determinationquality control
Publication Date：2025-01-29
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：6( 199-204 )