Optimization of the processing technology of Artemisiae extract by Box-Behnken response surface method and its quality control
WANG Yue
WANG Ya-qian
DING Yin
GOU Xiao-ling
HUANG Xue-mei
XIE Yu-chen
ZHANG Jing
FAN Gang
Abstract：Objective:To optimize the processing procedure of Artemisiae extract,and to establish its HPLC fingerprint. Methods:The content of four components ( chlorogenic acid,rutin,isochlorogenic acid A,and isochlorogenic acid C) in Artemisiae extract were determined by HPLC. Single factor experiment combined with Box-Behnken design-response surface method was used to investigate the three factors:solid-liquid ratio,decoction time,and decoction times. The CRITIC weight assignment method was used to calculate the weight coefficients of the four components and obtain the comprehensive score,which was used as the evaluation index to optimize the processing technology of Artemisiae extract. Moreover,the chemical fingerprint of Artemisiae extract was established by HPLC,and the similarity evaluation was performed by"Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2012 Edition)". Results:The best processing procedure of Artemisiae extract is adding 10 times the amount of water,decocting for 3 times,decocting for 1.6 h each time,and then combining the filtrate and concentrating into a thick extract. Three batches of samples were prepared for verification. The average comprehensive score was 94.69,and the RSD was 0.56％,which was close to the predicted value,indicating that the processing technology was feasible. The average contents of chlorogenic acid,rutin,isochlorogenic acid A and isochlorogenic acid C were 2.23％,0.41％,0.66％ and 1.39％,respectively. A total of 10 common peaks were identified with the similarity ranging 0.965 to 0.998 . The similarity of all samples was greater than 0.95,meeting the requirements of fingerprint research,which indicated that the similarity of different batches of samples was good. Conclusion:The optimal processing technology determined in this study is stable and feasible. The established methods of content determination and HPLC fingerprint are accurate and reliable,which provides a reference for the quality control of Artemisiae extract.
Keywords：Artemisiae extractBox-Behnken design-response surface methodologyprocessing technologyHPLC fingerprintsquality control
Publication Date：2025-01-14
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：8( 94-101 )