Study on UPLC fingerprint and multi-component content determination of Kemin capsules
DUAN Long-yun
CHEN Wen-jing
YANG Zong-tong
SUN Peng
LIU Shan-xin
YU Bei-bei
Abstract：Objective:To establish the UPLC fingerprint of Kemin capsules and screen its quality markers with chemical pattern recognition,so as to improve the quality standard of Kemin capsules.Methods:UPLC fingerprints were established for 25 batches of Kemin capsules,chemical pattern recognition[principal component analysis(PCA),hierarchical cluster analysis(HCA),orthogonal partial least squares-discriminant analysis(OPLS-DA)]was used to distinguish different origins and batches of Kemin capsules,and quality markers were screened.Results:The similarity of 25 batches of Kemin capsules was greater than 0.9,a total of 23 common peaks were calibrated,and 12 difference markers were screened by OPLS-DA method.Nine components including ephedrine hydrochloride,pseudoephedrine hydrochloride,methylephedrine hydrochloride,liquiritin,benzoylmesaconine,isoliquiritin apioside,benzoylaconine,benzoylhypacoitine,and ammonium glycyrrhizinate were identified as the quality markers of kemin capsules and the determination methods were established.The linear ranges were as follows:3.63～6.74,3.44～11.74,0.83～2.12,3.32～7.78,0.18～0.83,0.44～1.18,0.19～1.24,0.47～2.77,5.52～16.36 mg·g-1.Conclusion:The combination of fingerprinting,simultaneous determination of multi-component content and chemical pattern recognition can provide a more comprehensive evaluation of Kemin capsules and a theoretical basis for its pharmacodynamic material basic research and clinical application.
Keywords：Kemin capsulefingerprintschemical pattern recognitionquality markercontent determinationquality evaluation
Publication Date：2024-11-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：10( 2390-2399 )