Xuefu Zhuyu decoction decreases the risk of bleeding induced by different antithrombotic drugs

LIU You-chen; LIN Liu-qing; ZHANG Xiu-bing; XIE Hui-yi; ZHENG Yu-ying; LIU Fang-le; ZHAN Ya-xian; LIN Bao-qin

doi:10.3969/j.issn.1003-3734.2024.21.014

Abstract：Objective:To investigate the effect of Xuefu Zhuyu decoction(XZD)on bleeding induced by different antithrombotic drugs.Methods:The effect of XZD on the clotting time was observed by the capillary glass tube method in KM mice 1 h after a single administration.The effect of XZD on bleeding time in KM mice was determined by tail-tip amputation in KM mice 1 h after the final dose of 4 consecutive days.The combination of the traumatic blood stasis(TBS)model and warfarin was established in rats.After administration of XZD for 4 consecutive days,leg swelling was detected using the glass volume method,and ecchymosis was recorded 1 h after the final administration.The bleeding models induced by warfarin or low molecular weight heparin(LMWH)combined with aspirin were established in mice.After administration of XZD for 4 consecutive days,the bleeding time,activated partial thromboplastin time(APTT),and prothrombin time(PT)were measured 1 h after the final administration,and the international normalized ratio(INR)was also calculated.Results:Compared with the normal group,XZD prolonged clotting time 1.49-fold without affecting bleeding time.Concurrently,XZD reduced ecchymosis and leg swelling in rats treated with warfarin combined with TBS.XZD significantly decreased bleeding time by 47.07％,APTT by 35.54％,and PT by 29.53％in mice with warfarin-induced bleeding,while converting INR from a high bleeding risk range to a safe range.XZD significantly reduced bleeding time by 63.51％in mice with combination of LMWH and aspirin-induced bleeding and APTT unchanged.Conclusion:XZD reduced the risk of bleeding induced by different antithrombotic drugs.