Screening of anti-myocardial cell apoptosis active components in the seeds of Lepidium apetalum Willd
LI Jing-yang
ZHANG Li
YANG Fang-fang
LU Ren-rui
LI Meng
FENG Wei-sheng
ZHENG Xiao-ke
Abstract：Objective:To screen the components with ameliorating effects on doxorubicin-induced cardiomyocyte injury model among the 27 compounds obtained by extraction and isolation from the seeds of Lepidium apetalum Willd.and conduct preliminary studies on their mechanisms of action.Methods:The optimal concentration of doxorubicin was screened,5 μg·mL-1 was used to establish H9c2 cardiomyocyte injury model,and the cells were treated with compounds in the seeds of Lepidium apetalum Willd.for 24 h,then cell viability,LDH,T-SOD,MDA,GSH-Px levels were measured.ROS,mitochondrial membrane potential and apoptosis rate were detected by flow cytometry assay.In-Cell-Western assay was used to detect the expression levels of key proteins in the mitochondrial apoptotic pathway.Results:Lepidiumoside D(LS-18),quercetin3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside(LS-22),quercetin-3-O-β-D-galactopyranoside(LS-26)from the seeds of Lepidium apetalum Willd.could effectively enhance cell viability,reduce LDH,MDA levels,increase T-SOD,GSH-px levels,and improve oxidative stress.Further research showed LS-18,LS-22,LS-26 could decrease cell ROS level,enhance mitochondrial membrane potential level,inhibit apoptosis rate of cardiomyocytes and the expression level of the key protein Caspase-3 of the mitochondrial apoptosis pathway,and reduce the level of Bax/Bcl-2.Conclusion:Components LS-18,LS-22,LS-26 from the seeds of Lepidium apetalum Willd.have significant protective effect on H9c2 cardiomyocyte in doxorubicin-induced injury,and the mechanism may be related to improve oxidative stress homeostasis and inhibit the mitochondrial apoptotic pathway.
Keywords：the seeds of Lepidium apetalum Willdlepidiumosidedoxorubicincardiomyocyte injurymitochondrial apoptosis pathwayoxidative stress
Publication Date：2024-09-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：10( 1933-1942 )