Preparation and quality evaluation of antihypertensive candidate drug 221s(2,9)lipid emulsion
CHEN Yun-mei
YANG Kuan
YU Li-li
WANG Rong
TANG Yi-mei
QIN Bei
Abstract：Objective:To study the optimum preparation conditions of 221s(2,9)lipid emulsion(221-LE)and evaluate its quality.Methods:221-LE was prepared by two-step emulsification method.The particle size and polydispersity index(PDI)were taken as evaluation indexes to determine the formulation and preparation process.Results:The optimal prescription and preparation were as follows:by mass percentage,the compositions of long-chain triglyceride:medium-chain triglyceride(1∶4),egg yolk lecithin,F68 and oleic acid were 10%,2%,0.1%and 0.05%,respectively.The high shear rate of colostrum was 15 000 r·min-1 and high shear time was 5 min.The homogeneous pressure was 80 MPa,homogeneous cycle time was 10 times.221-LE,with the particle size of(131.50±1.53)nm,PDI of(0.12±0.01),Zeta potential of-(27.87±0.73)mV and entrapment efficiency of over 85%,had no hemolysis effect.Conclusion:The prescription process of 221-LE is stable and the quality is controllable,which provides a new perspective for the preclinical study of 221s(2,9).
Keywords：lipid emulsionantihypertensive candidate drug 221s(29)process optimizationquality evaluation
Publication Date：2024-08-31
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：9( 1719-1727 )