Analysis of chemical constituents in Gymnema sylvestre(Retz.)Schult via ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry
ZENG Zhi-hao
XIAO Guan-lin
JIA Can-chao
JIANG Jie-yi
TANG Rui-yin
JIA De-zheng
LI Yang-xue
LI Shao-lin
BI Xiao-li
Abstract：Objective:Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)was used to analyze the chemical constituents of Gymnema sylvestre(Retz.)Schult.Methods:A Waters CORTECS UPLC T3(150 mm ×2.1 mm,1.6 μm)column was utilized to separate the components using the mobile phase at a gradient elution of 0.1％formic acid-acetonitrile and the effluent was detected in electrospray anionic mode with the detection range of m/z 50～2 000.Results:Combined with the databases,literature,and reference standards,we identified 101 components,including 53 triterpenoids,37 flavonoids,5 organic acids,3 monoterpenoids,1 diterpenoid,1 phenanthrene,and 1 steroid based on the analysis of high-resolution mass spectrometry data(primary mass spectra and secondary mass spectral fragments).Conclusion:The chemical constituents of Gymnema sylvestre were identified using the fast,accurate,and simple UPLC-Q-TOF-MS/MS method for qualitative identification,which laid the foundation for establishing a quality standard of the herb and its material basis research.
Keywords：Gymnema sylvestreultra-performance liquid chromatography quadrupole time-of-flight mass spectrometrychemical constituentstriterpenoidsflavonoids
Publication Date：2024-08-15
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：13( 1595-1607 )