The effect of Lezhe polysaccharide crude extract on inhibiting H2O2-induced oxidative damage in HepG2 cells in vitro and its mechanism
FAN Hui-fang
LI Guo-feng
CHEN Hai-fang
ZHANG Wu-gang
Abstract：Objective:To investigate the in vitro antioxidant effect and mechanism of LZC based on ROS scavenging system and H2 O2-induced oxidative damage model in HepG2 cells.Methods:The scavenging effect of LZC on hydroxyl radical,superoxide anion,DPPH radical and nitrite ion were determined.The levels of MDA and GSH-Px in H2 O2-induced oxidative damage model of HepG2 cells were detected by kits.The intracellular ROS levels were detected by DCFH2-DA fluorescent probe.The apoptosis was detected by Annexin V-FITC/PI double staining.The protein expression levels of Nrf2/HO-1,p53,Bax and Caspase-3 were detected by Western blot.Results:LZC showed strong ROS scavenging activity,significantly increased GSH-Px activity in H2 O2-induced oxidative damaged HepG2 cell model,and inhibited MDA level,ROS release and apoptosis.Western blot showed that LZC up-regulated the protein expressions of Nrf2 and HO-1,and downregulated the expressions of p53,Bax and Caspase-3.Conclusion:LZC has a strong antioxidant effect in vitro,which is related to the activation of Nrf2/HO-1 signaling pathway and the inhibition of apoptosis.
Keywords：Lezhe polysaccharide crude extractantioxidationnuclear factor erythroid-2-related factor 2/heme oxygenase-1 signaling pathwaycell apoptosis
Publication Date：2024-04-30
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：8( 810-817 )