Study on the mechanism of ophiopogonin D in improving pulmonary fibrosis based on proteomics
CHEN Ting
BAO Sheng-chuan
LI Jing-tao
WEI Hai-liang
HUI Yi
YAN Shu-guang
Abstract：Objective:To study the effect and mechanism of ophiopogonin D on pulmonary fibrosis in mice by proteomics.Methods:C56BL/6 mice were randomly divided into normal group,model group,positive control group and ophiopogonin D group according to body weight.Except the normal group,the other groups were given bleomycin hydrochloride 5 mg·kg-1 by tracheal drip,24 h later,the positive control group was given prednesone(5 mg·kg-1)by gavage,and the ophiopogonin D group was given ophiopogonin D(10 mg·kg-1)by gavage for 14 days.The imaging changes of lung tissues were observed by Micro-CT,and the degree of pulmonary fibrosis of mice was observed by staining.The differential proteins in lung tissues were detected by DIA quantitative proteomics technology,and the main pathways of enrichment of these differential proteins were analyzed.Western blot(WB)was used to verify the inflammation-related proteins in lung tissue.Results:Compared with the model group,the administration of ophiopogonin D could significantly improve the pathological status of pulmonary fibrosis in mice.CT showed less lung consolidation,staining indicated less lung collagen fibrosis,and the positive expression of α-smooth muscle actin(α-SMA)in lung tissue was reduced.Proteomics showed that compared with the model group,there were 65 different proteins in the lung tissue of ophiopogonin D group,among which 46 proteins were up-regulated and 19 proteins were down-regulated.GO functional enrichment was found,differential proteins regulated by ophiopogonin D were involved in biological processes,molecular functions,and cell components,and were mainly concentrated in response to stress,carbohydrate derivative binding and myofibril.KEGG analysis showed that the signaling pathways involved in ophiopogonin D mainly included coronavirus disease-COVID-19,necroptosis,complement and coagulation cascades,NOD-like receptor signaling pathway,TNF signaling pathway and IL-17 signaling pathway.WB results showed that ophiopogonin D could significantly reduce the expression of NLRP3,IL-18,TNF-α and IL-6(P＜0.001,P＜0.01).Conclusion:Ophiopogonin D can improve fibrosis induced by bleomycin,and its mechanism may be closely related to reducing inflammatory response and regulating these pathways.
Keywords：pulmonary fibrosisophiopogonin Dproteomics
Publication Date：2024-02-29
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：11( 372-382 )