Variation of four chemical components in plantaginis semen during decoction process
TIAN Wei
ZHEN Ya-qin
DONG Qiu-ju
ZHANG Chuang
CHEN Zhong
NIU Li-ying
Abstract：Objective: To study and analyze the changes of four chemical components ( geniposidic acid, caffeic acid, acteoside and isoacteoside) in plantaginis semen during decoction process. Methods: An HPLC method was developed. The method was carried out on a Kinetex C18 column ( 100 mm × 2. 1 mm, 2. 6 μm) eluted with the mobile phases of 0. 5％ acetic acid and acetonitrile in gradient mode. The flow rate was 0. 3 m L·min-1 and the column temperature was set at 30 ℃. The wavelength was set at 239 nm for geniposidic acid, 325 nm for caffeic acid, and 330 nm for acteoside and isoacteoside. The contents of four chemical constituents in herbs, decoctions and granules were determined. Results: During the preparation process, the four chemical constituents changed with the decocting time, temperature and p H value. Conclusion: During the decocting process, the cleavage of ester bond in acteoside may result in caffeic acid, and partial conversion to isoacteoside.
Keywords：plantaginis semendecoctionformula granulesgeniposidic acidcaffeic acidacteosideisoacteosidecontent determination
Publication Date：2018-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 1927-1931 )