Pharmacokinetics of new compound RY10-4 in rats

LIU Zi-wei; RUAN Jin-lan; WANG Xing-xin; WU Wei; WANG Ying; ZHANG Heng; WEI Han

Abstract：Objective: To establish an HPLC analytical method of a new compound RY10-4, and evaluate its pharmacokinetics in rats. Methods: The concentrations of RY10-4 were determined by HPLC-UV with mobile phase consisting of methanol-water ( containing 0. 2％ phosphoric acid). The plasma samples were extracted by50％ phosphoric acid aqueous solution-ethyl acetate and processed with vortex and centrifugation to extract the target compound from plasma. And then the specificity, linearity, precision and recovery were evaluated by using apigenin as an internal standard. SD rats were employed as the animal models to obtain the concentration-time curves. The concentration-time data were analyzed by DAS 2. 1. 1. Results: The HPLC method and plasma sample pretreatment both met the requirement for the experiment. The pharmacokinetic analysis by the DAS software showed that the pharmacokinetics of RY10-4 conformed to two-compartment model and first-order dynamics. RY10-4 was eliminated mainly through the central compartment, rather than diffusing into the periphery compartment. High concentration of RY10-4 was prone to cause accumulation of RY10-4 in plasma, thereby postpone the elimination of the drug. Conclusion: The established HPLC method for RY10-4 can be performed in mild conditions and hasextensive applicability. RY10-4 has short Tmaxand rapid elimination rate and is prone to accumulate at high concentrations in rats.