The analgsic effect and possible mechanisms of Geniposide on type Ⅱ collagen-induced arthritis in rats
JIN Gui-juan
YE Qi
XU Zhi-liang
Abstract：Objective:To investigate the analgsic effects of geniposide on collagen-induced arthritis (CIA) rats and its possible mechanisms.Methods:CIA rat was induced by injecting with Chicken type Ⅱ collagen in Freund's complete adjuvant and Freund's incomplete adjuvant,CIA rats were subjected to treatment with geniposide at 1,10 and 100 mg·kg-1 from days 18 to 24 after immunization,the analgesic effect of geniposide by measuring mechanical withdraw threshold (MWT).After behavioral test,the lumbar segments of spinal dorsal were dissected,the spinal dorsal horn glial fibrillary acidic protein (GFAP),a biomarker of astrocyte activation,were observed by Immunofluorescence staining and Western Blot.The levels of proinflammatory cytokines TNF-α and IL-1β were determined by ELISA.Results:10 and 100 mg·kg-1 geniposide significantly reversed CIA-induced mechanical evoked pain,CIA rat displayed an increasement in GFAP is attenuated by geniposide,as well as the levels of proinflammatory cytokines TNF-α and IL-1β.Conclusion:Geniposide displayed a significant analgesic effect on CIA-induced RA rat,the possible mechanisms may involve the inhibition of astrocyte activation and reduce proinflammatory cytokine production in the spinal cord.
Keywords：geniposiderheumatoid arthritispainastrocytes
Publication Date：2018-02-15
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 356-360 )