Determination of equilibrium solubility and apparent oil/water partition coefficient of berberine α-hydroxy β-decanoylethyl sulfonate
LIU Man
LI Hui
DING Jin-song
Abstract：Objective:To determine the equilibrium solubility and apparent partition coefficient (Papp) of berberine α-hydroxy β-decanoylethyl sulfonate (HB) in a series of phosphate buffer solutions (PBS) of different pHs to provide a theoretical basis for the exploitation of HB preparations.Methods:A high performance liquid chromatography (HPLC) method was established and used to detect the concentration of HB in water and buffer solutions;the partition coefficients of HB in n-octanol-water/buffer solution systems were determined by shaking flask method.Results:The equilibrium solubility of HB in water was 378.5 μg· mL-1 at 37 ℃ and the solubility was the lowest at pH 5.The Papp of HB was 0.096,and it was ＜ 1 at pH of ＞5 and ＞ 1 at pH of ＜5.Conclusion:The equilibrium solubility of HB and the apparent oil-water partition coefficient are related to the pH of the medium,suggesting that it is not easily absorbed after entering the gastrointestinal tract.
Keywords：berberine α-hydroxy β-decanoylethyl sulfonate (HB)equilibrium solubilityapparent oil/water partition coefficientHPLC
Publication Date：2018-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：4( 225-228 )