Experimental study of the effects of SKI on peritoneal fibrosis relating to peritoneal dialysis

WANG Yu-xun; AN Ya-chen; JIANG Yan-ru; LI Yan-zheng

Abstract：Objective:To investigate the effects of Shenkang injection (SKI)on peritoneal form、function and the expression of E-cadherin、α-SMA in mice model of peritoneal dialysis and its potential mechanism of prevention peritoneal fibrosis.Methods:Mice model of peritoneal fibrosis was induced by high glucose dialysate (4.25％,Baxter).The mice were randomly divided into five groups:control group,4.25％ PDS model group,4.25％ PDS + SKI low,middle and high dose groups.Control mice (n =8):received no treatment;model group (n =24):mice were treated with daily intraperitoneal injection of 4.25％ peritoneal dialysate (100 mL· kg-1 body weight);4.25 ％ PDS + SKI low (n =24),middle (n =24) and high (n =24) dose groups:mice were treated with daily intraperitoneal injection of 4.25％ peritoneal dialysate plus 4.5 mL· kg-1 SKI,9 mL· kg-1 SKI,18 mL· kg-1 SKI.At 2,4,6W of study,8 mice of 4.25％ PDS model group and 4.25％ PDS + SKI low,middle,high groups were randomly drew out and did experiment.Results:Normal peritoneums were covered by a layer flat mesothelial cell.Comparing with the mice in normal group,the peritoneums of the 4.25％ PDS model group were significantly thicker than the control group.Magnanimous collagen fibrils deposition,Ultrafiltration volume and endotoxin clearance rate were dramatic decline.Furthermore,were gradually deteriorated over time.Comparing with the mice in 4.25％ PDS modle group,the Peritoneal thickness of the 4.25％ PDS + SKI groups(include low,middle and high dose) had varying degrees of ease.Their ultrafiltration volume and endotoxin clearance rate were increased.The expression level of α-SMA in 4.25％ PDSmodel group was significantly higher than that in control group.Compared with the 4.25％ PDS model group,the 4.25％ PDS + SKI groups were significantly lower.Compared with the control group,the expression level of E-cadherin in 4.25％ PDS model group was decreased,the expression level of E-cadherin in 4.25％ PDS + SKI groups were increased than the model group.The changes of 4.25％ PDS + SKI the middle、high dose groups were the most significant for 4 weeks and 6 weeks.Conclusion:Shenkang injection may delay the progress of peritoneal fibrosis by inhibiting the transdifferentiation of peritoneal mesothelial cells,delay the progress of peritoneal fibrosis,and with the increase of the dosage and duration of treatment,the curative effect is more significant.