Effect of baicalin on the expression of NLRP3 inflammsome and inflammatory factors in monosodium urate-induced RAW264.7 macrophages
WEN Xue-ping
LIU De-jun
PEI Yi-xue
XU Ling-yun
Abstract：Objective:To explore the antigout mechanism of baicalin by investigating its effects on the expression of NLRP3 inflammatory and some inflammatory factors in monosodium urate-induced RAW264.7 macrophages.Methods:Cultured murine RAW264.7 macrophages in vitro were divided into control group,experimental drug groups which pre-received different concentrations of baicalin at final concentration of 2.5,5,10 or 20 μg· mL-1,respectively for 1 h before being induced by monosodium urate suspension at final concentration of 2 mg· mL-1,and model group which was treated by monosodium urate suspension at final concentration of 2 mg· mL-1.Twenty-four hours later the cells were collected and the mRNA expression of TNF-α,IL-1β,IL-18 and NLRP3 was determined by fluorescence quantitative PCR method and the levels of TNF-α,IL-1β and IL-18 in cell supernatant were detected by ELISA.Results:Compared with the control group,the gene expression of NLRP3,TNF-α,IL-1 β and IL-18 was significantly upregulated and the levels of TNF-α,IL-1 β and IL-18 were significantly increased by monosodium urate in the model group,and compared with the model group,the gene expression of TNF-α,IL-1β,IL-18 and NLRP3 and their contents were significantly inhibited in the baicalin-treated groups.Conclusion:Monosodium urate activates NLRP3 inflammasome and facilitates the excretion of TNF-α,IL-1 β and IL-18,and baicalin can significantly inhibit the phenomenon and has anti-inflammatory effect.
Keywords：baicalinmurine macrophage RAW264.7monosodium urateNLRP3 inflammasomeIL-1βIL-18TNF-α
Publication Date：2018-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：5( 190-194 )