Inhibiting effect of establishment of peripheral hyperinsulinemia on endogenous insulin secretion when using euglycemic clamp technique

LIU Hui; YU Hong-ling; LIU Jia-li; LI Jia-qi; TAN Hui-wen; YU Ye-rong

Abstract：Objective:To investigate whether euglycemic clamp without hyperinsulinemia can inhibit endogenous insulin secretion after administration of short-action insulin in comparison with euglycemic hyperinsulinemic clamp technique (EHCT) in Chinese healthy male volunteers.Methods:Forty-eight Chinese healthy male volunteers were randomly divided into two groups (group A for EHCT,group B for euglycemic clamp without hyperinsulinemia).The target glucose was kept at (4.5 ± 10％) mmol·L-1 in group A and 10％ less than fasting blood-glucose in group B.The decrease percentages of C-peptide at the time of 1,2,3,4,5,6,7 and 8 h after administration and the AUC were compared.Results:C-peptide decreased to different extents compared to its baseline after administration of short-action insulin in both two groups.Endogenous insulin secretion was inhibited to different degrees during the period of glucose clamp in the two groups.The differences of AUC of decline of C-peptide at the time of 1,2,3,4,5,6,7 and 8 h after administration of short-action insulin between group A and B were not statistically significant [AUC0-1h:(5.6±6.2) min*％ vs (8.5 ±6.5)min*％,P=0.054;AUCo-2h:(14.1 ±12.5) min*％ vs (21.2±14.9) min*％,P=0.097;AUC0-3h:(22.6±18.4) min*％ vs (31.6±21.4) min*％,P=0.159;AUC0-4h:(33.7±26.7) min*％ vs (39.5 ±26.8) min*％,P=0.365;AUC0-5h:(49.4 ± 36.6) min * ％ vs (47.3 ± 31.6) min * ％,P =0.875;AUC0-6h:(67.2 ± 45.5) min * ％ vs (58.8 ±37.6) min*％,P=0.808;AUC0-7h:(85.4±55.2) min*％ vs (70.1 ±44.2) min*％,P=0.593;AUC0-8h:(108.4±67.5) min*％ vs (78.6 ±49.6) min*％,P =0.353].Conclusion:Euglycemic clamp without hyperinsulinemia can inhibit endogenous insulin secretion after administration of short-action insulin,which may describe the PK/PD characteristics of insulin preparations more accurately,and is more convenient and time-saving.