Preparation of Cleome gynandra cataplasm and study on its anti-inflammatory and analgesic activity

LIU Hui-juan; ZOU Chuan-zong

Abstract：Objectives:To study the cataplasm preparation of the overground parts of Cleome gynandra L.,screen the optimum composition and proportion.And the anti-inflammatory and analgesic activities in vivo were tested.Methods:The optimum composition and proportion was sieved by taking single factor and orthogonal experiments,using the initial adhesion,uniformity,spreadability,tracing ability of skin,bubbles and transmittance to nonwoven cloth as index of quality assessment.The anti-inflammatory effect on the formaldehyde-induced tumefaction of rats' toes was observed,and the licking time of mice was studied.Results:The optimal formulation was composed by 1.0 g of CMC-Na,0.8 g of gelatin,0.5 g of polyvinyl alcohol (PVA) and 2.0 g of aerosol.When drug-loading rate is 40％ of matrix amount and glycerol is 1/3 of the total volume of water using by matrix,the C.gynandra cataplasm has good viscidity.The time of decreasing swelling of toe was shorter,and the pain threshold of mice was raised.There are significant differences between treated group and the control group.Conclusion:C.gynandra cataplasm which is provided to possess smooth appearance and qualified assessment could be used to relieve the arthritic swelling and pain,because it is proved to have prominent anti-inflammatory and analgesic activities.