Inhibition effects of Pt(Ⅳ) on proliferation of breast cancer cells
QIN Yan
ZHAO Ming-hui
HUO Shu-ying
SHI Yan
WEI Tian-zhu
HUANG Sheng-nan
LIU Yong-lian
LIU Xiao-qing
NIE Zi-yuan
SHI Jian-hong
Abstract：Objective:To investigate the effect of Pt (Ⅳ) on proliferation and apoptosis of breast cancer cells (MCF-7 cells).Methods:Cell count array,MTS assay and FCM were conducted to assess cell viability,proliferation and apoptosis.Western blotting analysis was used to assay the effect of Pt(Ⅳ) on expression of apoptosisrelated proteins,and the key proteins in the PI3K/Akt and MAPK pathways.Results:Pt (Ⅳ) significantly reduced survival and inhibited proliferation of MCF-7 cells in a dose-dependent manner.Furthermore,Pt (Ⅳ)decreased protein expression of Bcl-2 and increased the expression of active caspase 3 and Bax in MCF-7 cells.Pt (Ⅳ) down-regulated the levels of p-Akt and p-ERK1/2.Conclusion:Pt(Ⅳ) has multiple antitumor effects including inhibition of cell survival and promotion of apoptosis in breast cancer cells.Suppression of p-Akt and p-ERK in the PI3K/Akt and MAPK pathways may be involved in its effect on proliferation and apoptosis of breast cancer cells.
Keywords：Pt(Ⅳ)breast cancer cellsproliferationapoptosissignal pathways
Publication Date：2018-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：9( 69-77 )