Effects of total saponins from rhizoma dioscorea nipponica on biomarkers in serum of gouty arthritis rats

LIN Fang-fang; LIU Shu-min; ZHOU Qi; ZHANG Na

Abstract：Objective:To explore the effects of total saponin of rhizoma dioscorea nipponica (RDN) on contents of uric acid,creatinine,lactic acid,glycine and tryptophan in serum of gouty arthritis in rats by high performance liquid chromatography(HPLC) and UV spectrophotometric.Methods:60 Wistar rats were randomly divided into six groups.They were normal group,model group,total saponins groups of high (160 mg· kg-1),middle (80 mg·kg-1) and low (40 mg·kg-1) doses and colchicine group.Total saponins of RDN were given for 7 successive days.An hour after total saponins of RDN were given at the 3 d,0.2 mL 25 mg· mL-1 MSU suspension was injected into articular cavity through anadesma of kneecap in the knee-joint to induce model of gouty arthritis.A Diamonsil C18 column(250 mm ×4.6 mm,5 μm) was used for the HPLC analysis at 30 ℃,The separation was carried out with the mobile phase consisting of methanol:acetonitrile (4:6)-ammonium acetate (0.03 mol· L-1) at a flow rate of 1 mL·min-1 The eluates were monitored by the wavelength at 360 nm for glycine and tryptophan;The contents of uric acid,creatinine and lactic acid in serum were determined by UV spectrophotometric.Results:In the model group,the levels of glycine and tryptophan were significantly decreased and the contents of uric acid,creatinine and lactic acid were significantly increased.Compared with the model group,glycine and tryptophan increased significantly while uric acid,creatinine and lactic acid decreased significantly in total saponins groups.Conclusion:Total saponin of RDN have potential effects on the treatment of gouty arthritis by regulating the content changes of glycine,tryptophan,uric acid,creatinine and lactic acid.