Uncertainty evaluation of determination of ceritinib concentration in plasma by LC-MS/MS
DU Ping
LI Peng-fei
ZHAO Rui
LIU Hong-chuan
LIU Li-hong
Abstract：Objective:To evaluate the uncertainty of determination of ceritinib in plasma by LC-MS/MS method.Methods:The uncertainties caused by various factors in the whole process of determination,such as repeatability,weighing,solution preparation,sample preparation process,the apparatus and calibration fitting,were comprehensively estimated.The uncertainty caused by random effects and others was elucidated in terms of type A and type B,respectively.The expanded uncertainties and combined uncertainty were analyzed with all the components.Results:The expanded uncertainties for ceritinib concentrations of 10,100 and 800 ng·mL-1 were 13.122,13.397 and 125.654 ng· mL-1,respectively (P =95％,k =2).Conclusion:The uncertainty of this LC-MS/MS method is mainly caused by calibration curve fitting.
Keywords：ceritinibuncertaintyLC-MS/MS
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 2719-2725 )