Protective effects of Qianggushengxue oral liquid on chemotherapy-induced bone marrow suppression in mice

LUO Gui-fang; TANG Ya-hui; WU Li-feng; LIU Yue; XU Zhi; ZENG Gui-rong; XIN Hong-ya

Abstract：Objective:To investigate the protective effect of Qianggushengxue oral liquid (QGSX) on chemotherapy-induced bone marrow suppression in mice.Methods:A total of 192 BALB/c mice with body weight of 20 ·25 g were randomly divided into 6 groups:sham,model group,three QGSX-treatment groups of different doses (3.9,7.8,15.6 mL· kg-1) and a positive drug group treated with Dangguibuxue oral liquid (2.6 mL·kg-1).All animals were orally treated with respective drugs or water once each day for 31 d.In the period of the experiments,except the normal group,bone marrow suppression were induced in the animals in the other 5 groups by combined treatment with acetylphenylhydrazinem (APH) and cyclophosphamide (CP).On the 1 lth,18th,25th and 32th days,8 animals in each group were executed respectively to collect blood samples for blood routine examination and to gather femoral bone marrow for nucleated cell count and cell cycle.The contents and protein expression of vascular endothelial growth factor (VEGF) and platelet endothelial cell adhesion molecules (PECAM-1)were examined by ELISA and Western blotting respectively in femoral bone marrow.Results:QGSX could significantly increase the content of HGB in normal mice and inhibit the decrease in the contents of HGB,PLT and RET ratio in APH-CP-treated mice.Also,QGSX could attenuate the decrease in nucleated cell count and promote the transformation of nucleated cells into S and G2/M cycles.Furthermore,QGSX could increase both the contents and the protein expression of VEGF and PECAM-1 in APH-CP-treated mice.Conclusion:QGSX exhibits protective effects on chemotherapy-induced bone marrow suppression in mice,and its actions are related to the increase in the content of cytikines and modulation of bone marrow microenvironment.