Preparation and in vitro quality evaluation of salmon calcitonin phospholipid complex
FU Hai-juan
WANG Cai-yun
ZHANG Ying
GAO Yan
Abstract：Objective:To study the preparation technics of salmon calcitonin phospholipid complex (sCT-PC) and its physicochemical properties.Methods:Using composite rate as evaluation criterion,single-factor test and orthogonal experimental design [L16 (4)5] was adopted to optimize the main influential factors.The oil-water partition coefficient,infrared signature,crystal phase transition characteristics and other physicochemical characteristics of sCT-PC were determined.Results:The optimal preparation conditions of sCT-PC were:the ratio of sCT to PC was 1 ∶ 15,the reaction solvent,temperation and time were dichloromethane-ether (V/V =6∶ 4),25 ℃ and 4 h,respectively.The solubility and partition coefficients in n-octanol of sCT-PC had been significantly improved than sCT or the mixtures.Infrared spectrophotometry (IR),X-ray diffraction (XRD),differential scanning calorimetry (DSC) were used to verify the formation of sCT-PC.Conclusion:The formation of sCT-PC was greatly affected by the reaction solvent,reaction time and the ratio of drug to lipid.The lipophilic of sCT-PC significantly enhanced compared with sCT,which layed foundation for the further researching on its nanoparticles preparation.
Keywords：salmon calcitoninphospholipid complexorthogonal designcomposite ratephysicochemical properties
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 2602-2608 )