In vitro release and percutaneous permeability of paeoniflorin ethosomal gel

YANG Chang; FENG Jian-nan; DU Shou-ying; BAI Jie; WU Hui-chao; MA Jun-ming

Abstract：Objective:This study aimed to optimize the prescription of paeoniflorin ethosomal gel and study its release and permeation behavior.Methods:Uniformity,character,stability and pH value were taken as indexes of the quality evaluate to optimize the dosage of carboxymethylcellulose sodium(CMC-Na),glycerol and ethosome in the preparation prescription;Bag filter method and improved Franz diffusing cells were used to estimate the in vitro release and dermal permeability of ethosomal gel.High performance liquid chromatography (HPLC) was applied to detect the concentration of paeoniflorin in transdermal receiving solutions and the curve of paeoniflorin cumulative permeation and release amount versus time was plotted.Results:The optimal formulation gained from experiments was composed of 1.82％ CMC-Na,27.3％ glycerol and 36.4 mL· 100 g-1 ethosome;The release of paeoniflorin from ethosomal gel fitted first grade equation,which was a multiple process of diffusion,dissolution and corrosion,and accumulative releasing ratio within 14 h was (49.73 ± 1.79)％;Percutaneous accumulative penetration amount and rate of paeoniflorin were respectively (361.67 ± 69.98) μg· cm-2 and (19.40 ± 3.75)％.Conclusion:Gel material hinder slightly the release of paeoniflorin but the permeation rate and efficacy of paeoniflorin were improved significantly carried by ethosomal gel system,which had an important significance for the further research and development of effective,safe and novel delivery drug system.