Application of tissue autofluorescence in treatment of neonatal rats with hypoxic ischemic brain damage
ZHANG Xiao-dian
LI Hong-hai
TIAN Shu-hong
TAN Peng
FU Jian
Abstract：Objective:To explore the application of tissue aut of luorescence in the treatment of neonatal rats with hypoxic-ischemic brain damage.Methods:60 SD neonatal rats were randomly divided into sham operation group,electric stimulation group and model group,and each group with 20 rats.The hypoxic ischemic brain injury model of neonatal rats was made by Rice-Vannucci,and the autofluorescence of brain tissue of neonatal rats was detected by caliper small IVIS live imaging apparatus.By fluorescence detection,the changes of autofluorescence intensity in the brain tissues of the model group,the electric stimulation group and the sham operation group were compared by fluorescence detection.Results:The test results from the complete local and section three groups of neonatal rat brain tissue showed spontaneous fluorescence signal injury increased significantly,the most serious damage area and the degree of the rats in the model group,the brain tissue autofluorescence intensity increases respectively in sham operation group,model group and stimulation group.Conclusion:The stronger the fluorescence intensity is,the more serious the damage is.Electric stimulation therapy can reduce the area and extent of injury.This study provides a new method for the study and evaluation of electrical stimulation on the hypoxic-ischemic brain damage in neonatal rats.
Keywords：neonatal ratbrain tissueautofluorescencehypoxic ischemic brain damagein vivo imaging system
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：6( 2578-2583 )