Analysis of O-acetyl content of groups A, C, Y and W135 Neisseria meningococcal capsular polysaccharides by nuclear magnetic resonance
LI A-ni
YUAN Fei
MA Yu
WANG Jing
KONG Su-juan
XIE Gui-lin
Abstract：Objective:To determine the O-acetyl content of groups A,C,Y and W135 Neisseria meningococcal capsular polysaccharides (GAMP,GCMP,GYMP,GWMP) by nuclear magnetic resonance (NMR) method.Methods:After being processed with dissolving,centrifuging and transferring,the sample of Neisseria meningococcal capsular polysaccharides was monitored by NMR,and the test parameters such as D1,NS and calculating standards were optimized to meet the requirements of quantitative determination.Results:The detecting condition was determined to be as follows:the D1 values of GAMP was 18,and those of GCMP,GYMP and GWMP were all 20;the NS values of GAMP,GCMP,GYMP and GWMP were all 128;the calculating standard of GAMP was comparing the ratios of H-3 and H-4 proton signals with H1 signal,and that of GCMP was comparing the ratios of H-7 and H-8 proton signals with H-3e signal.The calculating standards for both of GYMP and GWMP were comparing the ratios of H-7 and H-9 proton signals with H-3e signal.Conclusion:The NMR method is simple,rapid and accurate.It is suitable for O-acetyl content determination of GAMP,GCMP,GYMP and GWMP.
Keywords：groups ACY and W135 Neisseria meningitidescapsular polysaccharidesO-acetyl contentnuclear magnetic resonance (NMR)
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 2417-2423 )