Preparation of erythrocyte membrane-camouflaged PLGA nanoparticles and preliminary investigation on their cytotoxicity and drug release in vitro
SUN Ya-nan
ZHANG Xiu-mei
MA Xin
QUE Xiao
SU Jing
QIU Ming-feng
Abstract：Objective:To prepare the erythrocyte membrane-camouflaged PLGA nanoparticles (RBC-NP),and to investigate their stability,drug release ability and cytotoxicity.Methods:RBC-NP and PLGA nanoparticles (PLGA NP) were prepared by nanoprecipitation and extrusion.The nanoparticles were characterized by Malvern ZS90 and transmission electron microscopy.DiO was loaded into two carriers,and their release characters in vitro were studied.CCK8 kit assay was used to detect the cell proliferation rates.Cytotoxicity of RBC-NP and PLGA NP was determined and compared.Results:The differences in particle size of three groups of RBC-NP and PLGA NP were 11.1 ～14.2 nm,and PDI scale of particles in different groups ranged from 0.131 to 0.155.Transmission electron microscopic pictures showed that the RBC-NPs had clear core-shell structure.The RBC-NP was stable in double distilled water or PBS for 6 days.RBC-NP showed a sustained release property compared to PLGA NP.The cytotoxicity of RBC-NP was significant lower than that of PLGA NP at 0.25,0.5 and 1 mg· mL-1.Conclusion:RBC-NPs have been prepared successfully,and shown lower cytotoxicity and sustained release ability in vitro.
Keywords：RBC-NPPLGA NPstructure characterizationdrug releasecytotoxicity
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：6( 2352-2357 )