Protective effects of Marsdeniatenacissima extract on CCl4-induced chronic liver injury in rats

SU Yuan; ZHANG Xiao-lei; ZHANG Li-jiu

Abstract：Objective:To investigate the protective effects of Marsdeniatenacissima extract on CCl4-induced chronic liver injury in rats.Methods:Fifty SD rats were randomly divided into the following groups:normal control group,model group,low-,middle-and high-dose M.tenacissima groups (10,20,40 mg· kg-1).CCl4 was given to rats by subcutaneous injection to make liver injury model.At the same time,the treatment groups were intragastricly administered with M.tenacissima twice a week for six weeks.The liver and spleen indexes of rats were calculated.The activities of ALT,AST in serum and SOD,MDA,GSH-Px and Hyp in liver tissues were tested.Histopathology of liver was observed by HE staining.The level of IL-2 in serum was tested by ELISA.The T lymphocytes (CD3 +,CD4 +,CD8 +) in blood were detected by using flow cytometer.Results:Compared with the model group,M.tenacissima-treated groups showed decreases in the activities of ALT,AST,SOD,MDA,GSHPx and Hyp,and an increase in IL-2 level.Moreover,the pathological damage of liver tissue was obviously improved by M.tenacissima.Furthermore,the proportions of CD3 +,CD4 +,CD4 +/CD8 + were reduced,while that of CD8 + cells was up-regulated.Conclusion:Marsdeniatenacissima extract has protective effect on CCl4-induced chronic liver injury in rats,which might be associated with its regulation of immunological function by counterbalance the T lymphocyte subsets.