Preparation and contents determination of the compound riboflavin in situ forming eye gel
XU Min
ZENG Rong
LEI Qun-fang
MA Wan-le
Abstract：Objective:To explore the formulation and establish an HPLC method for the determination of the compound riboflavin in situ eye Gel.Methods:Using the gel state under 25 ℃ and 35 ℃ respectively,and the release test results in vitro as the indicators to select the appropriate formulation;Developed an HPLC method to determine the contents of riboflavin sodium phosphate and vitamin C in the gel.Results:The optimum formulation contained the same volume of methyl cellulose (0.1％),glycerin (VG∶ VW =1.4∶1) and sodium citrate (9％);Riboflavin sodium phosphate and vitamin C show good linearity at 0.04 ～ 0.20 mg· mL-1 and 0.02 ～ 0.06 mg· mL-1,and the average recoveries were 100.34％ and 99.06％,respectively.The RSDs of precision were 0.055％ and 0.98％.Conclusion:The presented formulation is appropriate and the assaying method is stable and reliable.
Keywords：compound riboflavinvitamin Cin situ eye gelcontent determination
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：6( 1579-1584 )