A study on the hepatoprotective effect of Saussurea.hieracioides Hook.F.ethanolic extract against mice liver injury induced by ANIT

RAO Ya-qi; ZHANG Li; ZENG Jin-xiang; ZHU Ji-xiao; ZHONG Guo-yue; LUO Guang-ming; XIE Xiong-xiong; ZOU Hong

Abstract：Objective:To observe the protective effects of ethanolic extract of Saassurea.hieracioides Hook.F on mice liver injury induced by α-naphthyl isothiocyanate (ANIT).Methods:60 mice were randomly divided into 6 groups,including normal group,bifendate group,model group,the low,medium,and high dose group of ethanolic extract of Saussurea.hieracioides Hook.F (1.0,2.0,4.0 g· kg-1).The liver injury model was induced by lavage ANIT peanut oil solution.48 hours after modeling,the serum was collected to detect the level of alanine aminotransferase (ALT),aspartate aminotransferase (AST),total bile acid (TBA),total bilirubin (TBIL) and tumor necrosis factor-α (TNF-α).The 3 liver samples were collected for the examination of the contents of malondialdehyde(MDA),glutathione peroxidase (GSH-Px)and superoxidase dismutase (SOD)in liver homogenate,the analysis of the impact of mRNA TNF-α expression,and the observation of hepaticpathological changes.Results:Compared with the model group,the medium and high dose group of ethanolic extract significantly decreased ALT,AST,TBA,TBIL,TNF-α in blood serum(P ＜0.05,P ＜0.01).The low dose group only had evident effect on the TBIL,TNF-α in blood serum(P ＜ 0.05).All dose group remarkably advanced the activity of SOD and decreased the activity of MDA in liver homogenate (P ＜ 0.05,P ＜ 0.01).The low and medium dose group had significant effect on the GSH-Px level(P ＜ 0.01).The expression of mRNA TNF-α in mice with liver injury was affected by each dose group.All dose groups could ameliorate the hepatic pathological changes at different levels.Conclusion:the ethanolic extract of Saussurea.hieracioides Hook.F has a certain protective effect on mice liver injury induced by ANIT.