Effects of gastrodia capsule and gastrodin combined with carbamazepine on the hippocampal neurons and the expression level of mrp1 gene of PTZ-kindled mice

DANG Xiang-ji; WANG Yi-xuan; JIAO Hai-sheng

Abstract：Objective:This study was designed to illustrate the antiepileptic effects and possible mechanisms of gastrodia capsule and gastrodin combined with carbamazepine on PTZ-kindled mice,and further revealed the influence of gastrodia capsule and gastrodin on the antiepileptic action of carbamazepine.Methods:The states of epileptic seizure of amyl tetrazolium (PTZ)-induced epileptic mice were scored,to measure the antiepileptic effect of gastrodia capsule and gastrodin combined with carbamazepine.Then the general pathology and RT-PCR methods were used to study the effects of gastrodia capsule and gastrodin combined with carbamazepine on the hippocampus nerve cells and the expression level of mrp1.Results:The gastrodia capsule and gastrodin can significantly increase the antiepileptic effect of carbamazepine.The gastrodia capsule can significantly attenuate the damage of hippocampus CA3 pyramidal neurons,but gastrodin cannot.Further,the gastrodia capsule can dramatically revert the high expression level of mrp1 after multiple dosage of carbamazepine,on the contrary,the gastrodin cannot,and even further increase the expression level of mrp1 after multiple dosage of carbamazepine.Conclusion:The gastrodia capsule can significantly increase the antiepileptic effect of carbamazepine,and can also attenuate the damage of hippocampus CA3 pyramidal neurons and decrease the high expression level of mrp1 after multiple dosage of carbamazepine.Nonetheless,the underlying mechanism for antiepileptic activity of gastrodia capsule still needs further study.