Tissue distribution of the lotus alkaloids in rats determined by LC-MS/MS
YE Lin-hu
KONG Ling-ti
YAN Ming-zhu
CAO Fang-rui
CHANG Qi
Abstract：Objective:To establish an LC-MS/MS method for the analysis of tissue distribution of nuciferine (NF),N-nornuciferine (N-NF) and 2-hydroxy-1-methoxyaporphine (HMA) in rats.Methods:Total alkaloids were administrated intravenously (10 mg· kg-1) to rats and the concentrations of NF,N-NF,and HMA in biological samples were determined with established LC-MS/MS method.Results:The results showed that NF,N-NF and HMA could be quickly (within 5 min) distributed to various tissues,especially in the liver,lung,spleen and kidney with relative high concentrations,and decreased significantly in 6 hours in most tissues.Conclusion:The established method is simple and accurate,and can be used to study the tissue distribution of NF,N-NF and HMA in rats.The NF,N-NF and HMA are widely distributed and remained in the bodies for a relative long period.
Keywords：tissue distributionLC-MS/MSnuciferineN-nornuciferine2-hydroxy-1-methoxyaporphine
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 1503-1509 )