Effect of dendrobium polysaccharides on injury of endothelium-dependent relaxation induced by high glucose
ZHONG Hui-juan
CHEN Lu
ZHOU Jie
WANG Bo
Abstract：Objective:To investigate the effect of dendrobium polysaccharides (DP) on the injury of endothelium dependent relaxation (EDR) induced by high glucose of isolated thoracic aorta in Sprague-Dawley (SD) rats and explore the mechanism.Methods:Organ baths in thoracic aortic rings of SD rats were used to investigate the effect of different concentrations of DP on the injury of EDR of thoracic aorta rings induced by high glucose.The expressions of endoplasmic reticulum stress protein markers including glucose-regulated protein 78 (GRP78),CCAAT/enhancer-binding protein homologous protein (CHOP) and nuclear transcription factor kappa B (NF-κB) in the thoracic aorta rings were measured by Western Blot.Results:High glucose (44 mmol.L-1) inhibited significantly EDR mediated by acetylcholine in thoracic aorta rings of rats.High glucose did not affect non-EDR mediated by sodium nitroprusside in thoracic aorta rings of rats.The injury of EDR in thoracic aorta rings of rats induced by high glucose was inhibited by DP (200,400 and 800 μg· mL-1).High glucose increased significantly the expressions of GRP78,CHOP and NF-κB in the thoracic aorta rings (P ＜ 0.05).Compared with the high glucose group,the expressions of GRP78,CHOP and NF-κB in the thoracic aorta rings in high glucose + dendrobium polysaccharides (200,400 and 800 μg· rL-1) group were significantly decreased (P ＜ 0.05).Conclusion:Dendrobium polysaccharides inhibit the injury of EDR of thoracic aorta rings induced by high glucose,and the mechanisms may be related with the inhibition of endoplasmic reticulum stress and NF-κB signaling pathway.
Keywords：dendrobium polysaccharideshigh glucoseendothelium dependent relaxationendoplasmic reticulum stressglucose-regulated protein 78CCAAT/enhancer-binding protein homologous proteinnuclear transcription factor kappa B
Publication Date：2017-01-01
Online Publishing Date：2026-08-14(First online date of this platform, not the publication date of the document)
Pages：7( 1443-1449 )